Effects of grape Procyanidins on proliferation of hepatocytes in vitro and vivo

  Objective   To study the effectof GPC on the prolifer ation of normal hepatocytes and the cells damag ed byethanol.

  Methods   ① Normal primary mouse hepatocytes and the cells damag ed byethanol were cultrued with grape procyanidins(GPC)of different concentrations.Methyl thiazolyl tetrazolium(MTT)assay was used to analyze the cell proliferation.②GPC was given tonormal mouse and the ones with liverinjuried byethanol acutively through mouth.Cell proliferations of 3 h and 16 h were analyzed by the same way as above.

  Results   In vitro the proliferations of normal control and the ethanol group were 0.438±0.040 and 0.365±0.024 respectively.Those of the 50 mg/LGPC protective group and control group were 0.541±0.026 and 0.603±0.060 respectively.In vivo, the proliferation rates of the 150 mg/LGPC protective group were 0.095 higher than thatof the ethanol one.Thatof the 150 mg/LGPC control group was 0.030 higher than thatof the normal control one.All differences abo ve were sig nificand(P < 0.05).

  Conclusion   GPC can inhibitthe damage of ethanol on proliferation of hepatocytes, and promote proliferation of normal cells.