Effects and biomarkers of peroxynitrite on development of liver tumor

  Objective   To compare the DNA damages and cytotoxicit effect of perox ynitrite(ONOO^-)to human embr yoliver L-02 cells and human hepatic tumor HepG 2 cells.

  Methods   Twoliver cells were exposed to different concentration perox ynitrite(0.01, 0.05, 0.1mmol/L).Survival rate, level of superoxide dismutase(SOD), glutamy lcysteiny lgly cine(GSH), catalase(CAT), Malonyldiadehyde(MDA), DNA damage and the expression level of Growth arrestand DNA damag -einducible genes(GADD)were measured.

  Results   Afterexposed to peroxy nitrite, L-02 and HepG 2 cells survival rate decreased from 87.55%, 90.34% to 19.21%, 21.59% varying concentration ranging 0.01mmol/L to 0.2mmol/L, respectively.0.01mmol/L to 0.2mmol/L, respectively.Perox ynitrite caused GSH, CATlevel significantly change, decreased SOD level and increased MED level in L-02 and HepG2 cells(P<0.05).DNA damage and GADD expression level increased as perox ynitrite concentration increased in both two cell lines(P<0.05).All these damages were more serious in L-02 in every concentration level than thatof HepG2, butonly the expression level of GADD were significant difference between L-02 and HepG2 cells.

  Conclusion   Perox ynitrite had cytotoxicity effectand significant difference was observed in two cells.GADD expression level maybe one of the bio markers of NONN^- damage effects.