Identification and characterization of class 1 integron of Proteuse in meats food

  Objective   To explore the distribution and character of class 1 integrons in Porteues from meats, and to elucidate the status of gene-cassettes.

  Methods   Routine method was used to isolate Proteues and antibiotics susceptibility was tested by the disk diffusion method; class 1 integron was detected by PCR assay; PCR products were sequenced and analyzed.

  Results   Of 91 samples, 27 isolates were identified, and 13 isolates were multiple-drug resistant.The antibiogram was sulfamethoxazole-trimethoprim, ampicillin, streptomycin, tetracycline, erythromycin.3 of 14 isolates carried class 1 integrons; and two strains laboured two integrons with 1.8 kb and 0.75 kb, one strain laboured 110 kb integron.The sequenced PCR conduct demonstrated that the 118 kb integron laboured pse-1-aadA1-dfrA14 gene cassettes confered the resistance of β-lactamas, sulfamethoxazole-trimethoprim, and aminoglycoside; the 110 kb integron laboured aadA1 gene cassette confered the resistance of aminoglycoside.

  Conclusion   The different kinds of class 1 integrons exists in proteues from meats, and determines the multiple-resistant antibiotics.