Study on molecular epidemiology of poliovirus in Fujian province

  Objective   To study the molecular epidemiolo gy of poliov irus isolated from the feces of acute flaccid paralysis cases in Fujian.

  Methods   The order genetic fragment was amplified by RT-PCR and then sequenced.The result of the virus sequence was given to NCBIGenebank for contr ast,and co nstructed the phylong enetictrees.

  Results   5 poliovirus strains isolated in Fujian from 1999 to 2002 years,which were SabinⅡ and SabinⅢ-recombinant strains.The sequence analysis sho wed the identit ies of these 5 poliovirus strains and standerd vaccine was over 99%,ithad seldom difference in nucletide,and was seldom difference from Panzhihua strains.

  Conclusion   The vaccine-recombinant polioviruses had even been in Fujian from 1999 to 2002,one of the virus strain had already circled in human,and caused two cases.At present,our nation had already been polio-free,we must pay attention to vaccine-derived polioviruses strains when we still use OPV to immunize.The method of molecular epidemiology can provide us support to adopt crrelation strategy to prevent and control polio in time.