Effect of cadmium on apoptosis and expression of bcl-2, p53, c-myc, c-fos and ras genes in LLC-PK₁ cells

  Objective   To explore the effect of cadmium on the apoptosis of porcine renal prox imal tubule epithelial cells(LLC-PK₁)and the relationship between the apo ptosis and the bc-l 2, p53, -cmy c, c-fos and ras gene expression.

  Methods   The expression of bc-l 2, p53, c-myc, c-fos and ras were measured respectively by FCM.

  Results   After LLC-PK₁ cells were incubated with 0, 10, 20, 40 Lmol/LCdCl2 for4 h or8 h, the expression of bc-l 2, p53, c-myc and ras decreased and there was a significant dos-eresponse relationship(r=-0.910 of bc-l2 for 8 h, r=-0.716, -0.972 of p53 for4 h, 8h respectively, r=-0.694 of c-myc for 8h and r=-0.887 of ras for 8 h).

  Conclusion   The apoptosis of LLC-PK₁ cells could be induced by cadmium, and the inhibition of the expression of bc-l 2, p53, c-myc and ras would be one possible mechanism.