Objective To study the effects of selenium supplementation on related gene expression of liver in diabetic rats.
Methods cDNA fragments from former research project were cloned, sequenced and BLASTn analysed.The RT-PCR were made using the primers designed acco rding to the sequence of cDNA.
Results The identities between the sequence of Se-8 and protein phosphatase 2A(PP2A)was 99%.The expression level of DM group(0.507 2±0.057 4)and DM+Se group(0.698 2±0.039 6)was obviously lower than those of NC group(1.330 3±0.185 5)(P < 0.05).The ex pression level of DM+Se group(0.698 2±0.039 6)had some improvement compared with those of DM group(0.507 2±0.057 4)(P < 0.05).
Conclusion Selenium supplementation could improve the expression of PP2A mRNA on liver in diabetic rats.It may be one of the molecular mechanisms that Selenium could improve the disorders of glucose and lipids metabolism in diabetic rats.
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