Effect of antibactrial proteins of Musca domestica Larvae on A375

  Objective   To explore the inhibitory effects of the antibactrial pro teins from the Musca domestica larvae on the A₃₇₅.

  Methods   The effects of the antibactrial proteins from the Musca domestica larvae for the growth of the A₃₇₅ were observed by cytometry, MTT assay and acridium orange stainings.The effects of the antibactrial proteins from the Musca domestica larvae for the growth of the periodontal membrane cell of human were observed by MTT assay.

  Results   At the high-concentration groups, the cell number and the optical density(OD)of A₃₇₅ were significantly decreased, the apoptosis index (AI)was measured.With the increase of the concentr ation, the inhibitory rate of the cell proliferation werincreased.The OD and the inhibitory rate of the cell proliferation at the all concentration-groups had hardly difference compared with the control group in the periodontal membrane cell of human.

  Conclusion   The antibactrial proteins from the Musca domestica larvae might be inhibited the growth of A₃₇₅ by inducing cell apoptosis.The antibactrial proteins from the Musca domestica larvae could hardly inhibited the normal human cell.The inhibition effects had a threshold.