Systematic identification of human normal first trimester placental villus-derived trophoblastic cell line in vitro

  Objective   To set up a systematic method of character ization the human no rmal placental villus-derived trophoblastic cell line(HPT-8)in vitro.

  Methods   The human placental villus-derived trophoblastic cell line(HPT-8)in vitro was identified and assayed by light microscope, scanning electron microscope, transmission electron microscope, immunohistochemistry and immunofluorescence laser confocal microscopy.

  Results   Using the scanning electron microscope, there were abundance micro villi on cell sur face.Using the transmission electron microscope, there were abundance microvilli on cell surface.Using the transmission electron microscope, there was abundance ky to plasm.Endocy to plasmic reticulum distended expressly.There was desmosome structure beween the trophoblast cells.Keratin 18 and vimentin specifically bound to the cytoplasm of trophoblastic cells with directimmunofluorescence double marker.Keratin 7 and motility related protein-1 specifically bound to the cytoplasm or cytolemma of trophoblastic cells with immunohistochemistry.Epidermal growth fctor receptor, stromal cell derived fator and placental alkaline phosphatase were bound to the cytoplasm of trophoblastic cells with immunohistochemistry.Buthuman chorionic gonabotropin and placental lactogen didn't find in the cytoplasm of trophoblastic cells with immuno histo chemistry.

  Conclusion   HPT-8 cell line with 72 passage numbers in vitro was established from appearance, cystoskeleton and functional parameter.Pure trophoblastic cell line model is gained, which has part function of endocr ine secretion that could evolve empirical study in the future.