Construction and expression of recombinant plasmid and expression of fusion of HIV-1 env/IFNα-2b

  Objective   To construct an eukaryotic expression plasmid and express fusion of HIV-1 env/IFNα-2b in BHK 21 cell.

  Methods   An eukaryotic expression plasmid pSFJ16env/IFNα-2b gene into vaccine vector plasmid pSFJ16 and transfected to BHK 21 cell in vitro by liposome-mediated method.The expressed product was detected by indirect immunfluorescence assay(IFA), western blot and Dot-ELISA.

  Results   Indirect IFA showed green fluorescence on the surface of transfected cell.Both western blot and Dot-ELISA proved that the lysate of transfected cells contained env/IFNα-2b protein.

  Conclusion   An eukaryotic expression plasmid for HIV-1 env/IFNα-2b was successfully constr uced, and the expressed pro duct showed good reactinogenicity and immunogenicity.