Objective To develop enzyme-linked immunosorbant assay for detection of FB1 which is based on monclonal antibodies.
Methods To produce hybridoma cell lines excreting monoclonal antibodies against FB1 by using B cell hybridomatechnique and develop enzyme-linked immunosorbant assay for detection of FB1.
Results Hy bridoma cell lines excreting monoclonal antibodies were developed and monoclonal antibodies against FB1 were prepared.Monoclonal antibodies produced by the hybridoma cells were tested for subtypes and designated as IgG1, the mo lecular weight was 150kD, the titer was 1:2.0~108, the affinity constant was 6.72×109 L/mol.The limited concentration of detection was 5μg/L, linear range was 50~500 μg/L, the linearequation was Y=-0.582x+1.793, (r=0.99, P < 0.05).There was no cross reaction with DON, ZEN, T-2 toxin.The recovery rate of spiked maize was among 71.89% ~112.95%, and mean recovery rate was 100.23%.
Conclusion The detection method is simple, rapid, sensitive and can meet the demand of practical work.
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