Effects of excessive Vitamin A, Zn or both intaken on rat spleen

  Objective   To study the effect of excessive VitA, Zn or both intaken on lipid peroxidation and antioxidant status and cell cycle of normal adult rat spleen.

  Methods   The rat model was induced by lavaging solution containing VitA, Zn or both.After 2w and 3w, half numbers of every group were killed and measured the contents of total antioxidation pabilit y (T-AOC), the glutathione perox idase(GSH-Px), the malondialdehyde(MDA), the supero xide dismutase(SOD), and the cell cycle of spleen was detected by flowcytometry.

  Results   The T-AOC decreased significantly in every test group than the control after 3w.The SODlevel of the united gr oup and Zn group were significantly higher than the control.The expression in cell cycle blocked in the Sand G₂/M phase increased significantly in the VitA group and united group.

  Conclusion   Ex cessive VitA, Zn or both intaken could lead to a certain extent damage in rat spleen in short term, especially VitAand Zn both intaken.