Detection bacteria fragile by SYBR Green real-time PCR

ObjectiveTo identify the bacteria fragiles(B.fragiles)by 16Starget rRNA real-time PCR.MethodApair of specific primers was designed.The PCR products were detected by fluorescent quantificat ive real-time P CR.ResultsThe B.fragilis ATCC 25285 and 4 separative strains of B.fragilis appeared in the special amplification product curve,while E.coli, L.bulgaricus and S.thermophilus did not.The flourescent qantificative PCR can detect 10² bacteria.Conclusion16 SrRNA-targeted primer and fluorescent quantifive real-time PCRcan be applicable for the identification and quantification of B.fragilis.