Study on electrophysiological properties of K+ currents in Neutrophils

Objective To investigate the kinet ic proper ties of potassium channel in human neutr ophils.Methods Plasma membr ane K⁺ currents were studied using the cell-attached mode of patch-clamp method.Results Two distinct K⁺ currents obser ved was the voltage-dependent K⁺ current,its conductance was about 56pS;the other was voltage-independent,the amplitude of current was about-7pA.The channel was act ivated at -60mV holding potent ial,which was the resting membr ane potential of neurtophils.The open probability decreasd away from this holding potential.Conclusion Two distinct K⁺ currents including voltage-dependent and voltage-independent current are in the human neutrophils.There are close relationship between the potassium channels and the function of PMNS.