Selection of virus strain for production of inactivated hantavirus vaccine of vero cells

Objective To adapt the candidate strains of hemorrhagic fever with renal syndrome(HFRS)vaccine to Vero cells and to study their antigenicity and immunogenicity.Methods 4 strains of Hantavirus were continuously propagated in suckling mouse brains or in Vero cells and studied the antigenicity of virus,viral titers,the amounts of virus antigen and their immunogenicity.Results Four strains of Hantavirus adapted to Vero cells and stably grew in the cells or in suckling mouse brains with high titers.Rabbits immunized with the crude vaccines of ZJ4 and ZJ5 showed the neutralizing antibody titers of the rabbit immune sera reached 1B10 at 4 weeks after 2 times of immunization.Conclusion ZJ4 and Z J5 candidate strains adapted to Vero cells,possessed high titers with good immunogenicity and were feasible to prepare the HFRS vaccine of Vero cells.