Effects of environmental estrogen chemicals on VEGF and CXCR4 expressions in breast cancer of rats

Objective To study the effects of enviromental estrogen chemicals octylphenol(OP)and genistein(GEN)on the expressions of vascular endothelial growth factor(V EGF),V EGF receptor(flk-l)and CXC chemokine receptor type 4(CXCR4)in rat breast cancer.Methods Female Sprague-Dawley rats were randomly divided into control,model,GEN treated,OP treated and GP(GEN+OP)treated group.After 7,12-dinmethylbenzaanthracene(DMBA)induced rat breast cancers(Except rats in control group),rats were killed on day 210.Western blotting and immunohistochemistry methods were used to detect the expressions of V EGF,CXCR4 and flk-1 on normal mammary grand and mammary cancers.Results Compared with model group,V EGF,CXCR4 and ilk-1 expressions were significantly decreased in mammary cancers of genistein treated group,while they were significantly increased in octylphenol treated group.CXCR4 expression was significantly decreased in GP treated group.Conclusion GEN is down-regulate V EGF,CXCR4 and flk-1 expressions in mammary cancer then may inhibits the incidences of DMBA-induced mammary cancers.OP is up-regulate V EGF,CXCR4 and flk-1 expressions in mammary cancer then may increase the incidences of DMBA-induced mammary cancers.