Inhibiting effect of black tea polyphenols on proliferation of endothelial cell of artery

Objective To observe the effects of black tea polyphenols on endothelial proliferation and search for the mechanism of black tea polyphenols on inhibiting cell proliferation.Methods Endothelial cell proliferation was determined by MTT assay.P38MAPK phosphorylation was measured by Western blot.Results Black tea polyphenols inhibited cell proliferations in a dose-and time-dependent manner and block endothelial cell proliferation induced by PMA or AngⅡ(<0.05).Similar blocking effect can be achieved with NADPH oxidase inhibitor,apocynin or p38MAPK pathway inhibitor SB203580.Phosphorylation analysis showed black tea polyphenols postponed the transduction of p38MAPK phosphorylation induced by AngⅡ.Conclusion Black tea polyphenols inhibited endothelial proliferation mainly via restraining or postponing the PKC-NADPH oxidase-p38MAP Kpathway.