Typing of vibrio parahaemolyticus clinical isolated strains from Shenyang region by repetitive element sequence-based PCR

ObjectiveTo understand the epidemic and distribution characteristic of vibrio parahaemolyticus in Shenyang,analyze the typing possibility of vibrio parahaemolyticus by repetitive element sequence-based PCR.MethodsRepetitive Extragenic Palindromic PCR and Entero-bacterial Repetitive Intergenic Consensus PCR were used to generate DNA fingerprints for vibrio parahaemolyticus.Results40 clinical strains of vibrio parahaemolyticus,representing a wide range of DNA patterns,were grouped into 16 and 4 patterns,with discrimination indexes of 0.953 and 0.5 by REP-PCR and ERIC-PCR,respectively.Pattern G was predominant,accounting for 20.0% of total count,according to REP fingerprints,and pattern D was predominant,67.5% of total count,according to ERIC fingerprints.ConclusionRepetitive element sequence-based PCR can be used to type vibrio parahaemolyticus,which is super to the serotyping method.