Effect of high fat diet on Sirtl expression in fat tissue

Objective To observe the effect of hig h fat and high energy intake on silent information regulator1(Sirtl) expression in fat tissue.Methods High fat diet was adopted to stimulate obesity prone(OP)and obesity resist(OR)rat model,and RT-PCR was used to compare the expressive level of Sirtl mRNA.Results Body weight differed in high fat diet fed animals,OP >Control> OR,the difference was significant(P<0.05).Daily food intake and daily energy intake were significantly differ ent as well(P<0.05).OP animals had more body fat than OR and control animals(P<0.05).High fat and high energy intake decrease Sirtl mRNA expression in fat tissue.Conclusion Regulating Sirtl might be potent goal for preventing and treating obesity.