Establishment and evaluation of immunosuppressive model in mice influenced by cyclophosphamide

Objective To establish an immununosuppressive model in mice,to evaluate application of flowcytometry in health food evaluation.Methods Once injection with middle dose and intensification in proper intervals,daily inject ion with minor dose and once injection with large dose were used in establishing the model and an appr oved functional food was tested to validate the model.The main indexes used in the study were CD69⁺/CD3⁺;CD69⁺/NKG2D⁺;prolification capacity of lymphocyte(PCL)and NK cell activity.Results In once injection with middle dose and intensification in proper intervals,daily injection with minor dose groups,CD69⁺/CD3⁺,CD69⁺/NKG2D⁺ PCL and NK cell activity were significantly lower than those in the control group.Conclusion With once injection and intensificat ion in proper intervals method,a proper immunosuppr essive animal model for the functional food evaluating system can be established.CD69⁺/CD3⁺,CD.69⁺/ NI G2D⁺ has good susceptivity and application value in the immunity function evaluation of healtlh food.