Measurement of alveolar surfactants content after acrylamide-induced lung injury

Objective To observe the change of composition of PL and content of protein in PS after acrylamide damage of lungs.Methods The model of rat lung injury was made by intratracheally instilling acrylamide(0.1 mg/kg,0.4 mg/kg).24 rats were divided into four groups:122hour group,22day group,32day group,52day group.PS of each group were examined by electron microscope.Content of PL,composition of PL and content of protein of each group were determined respectively.Results Structure changes of PS were found in rats'lungs of experimental groups and 122hour group was more apparent.Content of protein in PS was the highest in 122hour group(55.61±9.97)and 122hour group was more apparent(180.4± 22.5).Conclusion Morphologic change and alternation of quality and quantity of PS after anatoxin2induced pulminary injury specifically reflect the process of lung damage.Content of PL and PI is a simple and feasible marker when lung injured by acrylamide in early stage in rat.