Clone and expression of Helicobacter pylori protein HP0410 and its epitopes analysis

Objective To study the feature of epitopes of HP0410-a surface protein of Helicobacter pylori by using phage display technology.Methods We first constructed an expression system of HP0410 in E.coli BL21 and then we used the purified recombinant protein to scan monoclonal antibodies of Hp and got one specific monoclonal antibody-E018.The antibody was used to construct a 12-peptides M13 phage library of HP0410.The DNA of positive phage was sequenced and the candidate was tested by competitive-inhibition array.The epitopes were analyzed by bioinformatics methods.Results We obtained 8 epitopes and a candidate epitope from the phage library by sequencing 13 phages DNA and analysis of bioinformatics.The competitive-inhibition array suggested that HP0410 could inhibit the binding between candidate phage and E018.Conclusion According to the bioinformatics analysis the epitopes may be the simulation of spatial epitopes of the high antigenicity segments and the candidate epitope is probably the simulation of antigen determinant specifically binding to E018.