Stndy on immunological properties of polysaccbaride extracted from rehmanniae preparata

Objective To extract polysaccharide from radix rehmanniae preparata,and to study its immunity activity.Methods Polysacchayide extracted from radix rehmanniae preparata by water-extraction-alcohol-precipitation method was determined by phenol sulfonic acid spectrophotometry.SRBC and ovalbumin employed as antigens were injected into the mice.Three doses of radix rehmanniae preparata polysaccharide50,100,200 mg/(kg·d)were given orally for seven days.After the secondary immunization,the level of corresponding antibody and the concentration of the serum IL-2 were determined.Results The contents of polysacchar ide of samples was 1.45% and the average recovery was 98.53%.Standard curve was liner in the concentration range of 5~40 μg/ml.The levels of antibodies(anti-SRBC and anti-ovalbumin)and cytokines(IL-2)in median dose group of radix rehmanniae preparata polysaccharide were all significantly higher than those of control groups.Conclusion Radix rehmanniae preparata polysaccharide could enhance the immune function in normal mice.