Effects of Ang-2 on intracellular Ca2+ i and mitochondria membrane potential in VEC

Objective To explore the effects of angiotensin-2 (Ang-2) on intracellular free calcium concentration and mitochondria membrane potential in vascular endothelial cell(VEC).Methods VEC were divided into control group and Ang-2 group.Intracellular free calcium concentration and mitochondria memborne potential were measured by laser scanning confocalm icroscopy.Results Intracellular free calcium concentration of control group was 24.781±1.350.In tracellular free calcium concentration of Ang-2 group was 47.384±1.323.Intracellular free calcium concentration of Ang-2 group was significantly higher than that of control group (P<0.01).Mitochondria membrane potential of control group was 36.578.14Z Mitochondria membrane potential of Ang-2 group was 9.016±1.124 and significantly lower than that of control group (P<0.01).Conclusion Ang-2 can induce obvious changes of intracellular free calcium concentration and mitochondria memborne potential of VEC.