Molecular characters of Vibrio cholerae pathogenicity island of Vibrio cholerae O139

Objective To compare molecular character of Vibriocholera epathogenicity island(VPI)of Vibriocholerae O 139 international reference strain MO 45 and ElT orbio type of Vibriocholerae international reference stra in N16961.Methods Segments including VC0815 gene in the upriver of VPI and it's upriver segment from MO 45 were screened and cloned to pNEBV C081545.Restriction map of pNEBV C081545 was drawn and compared with the map of the same segment of N16961.The pNEBV C081545 was subcloned and analyzed.Results There were several restriction enzyme sites in the upriver segment of VC0815 gene in the restriction map of pNEB-VC081545 including Kpnin at the 1st base pair,Saiv lat the 732nd base pair,Hpaiv at the 819th base pair,Bglòat the 1578th base pair,Hindòat the 1600 th ba se pair,X baiv at the 1943rd base pair,EcoRiv at the 3274th base pair.The restriction map of the segment was the same to the segment of N16961.The segment between Saiv land EcoRiv subcloned was the same to N16961.Conclusion The result of the study supports the view that Vibrio cholerae O 139 maybe results from the mutation of the O antagen of ElT or biotype of Vibrio cholerae.