Anti-inflammatory activity of anthocyanins from mulberry on adjuvant-induced arthritis

Objective To study antiox idant and anti-inflammatory activity of anthocyan ins from mulberry on adjuvant-induced arthritis(AA).Methods Sixty Spraque Dauleyrats were randomly allocated to six groups,except for the rats in the normal group,the rats were modeled to adjuvant arthritis with Freund′s complete ad juvant.The swelling degree of pawswere recorded and glutathione(GSH),malona ldehyde(M DA),supe roxide dismutase(SOD),and total antioxidative capacity(T-AO C)activity in serum were detected by special kits to assess the antiox idant effects of cyanid in,and the levels of NO in serum,activity of IL-1,TNF in serum and joint fluids of secondary arthritis were determined by radioimm unoassay.Results The mulbe rry anthocyanins could protect against the pawss welling in AA rats,increase the GSH-Px,SOD activity and T-AOC levels in serums and decrease MDA in model rats,and significantly decrease the activity of 1β(IL 21β),TN Fα and NO in serum and TNF α in joint fluids of secondary arthritis.Conclusion Mulberry an thocyan ins has a remarkable treatment effect on AA and the anti-inflamm ation effect might be via improving the total antioxidative capacity and scavenge the free radicals.