Induction of TR1 by sulforaphane in human immortalized hepatocytes

Objective To study the effect of sulforphane(SFN)on TR1 m RNA expression in human immortalized hepatocytes(HHL 25 cells)and the potential mechanism involved.Methods WST assay was used to observe the cyto toxicity of SFN.TR1 mRNA induction was quantified by TaqMan one-step RT-PCR and Nrf 2 expression was detected by Western blot.Results SFN promoted HHL 25 cells growth at 5μM and inhibited cells growth at higher dose.TR1 mRNA was induced dose-dependently by SFN(2～10μM 24h),with the maximal induction of 4162fold with 10μM SFN treatment.Moreover,Nrf2 prote in was up regula ted in a time-dependent manner both in cytop lasm and in nucleus after 20μM SFN exposure,and significant induction was shown at 1h.Conclusion SFN might increase an tioxidative defense in cells through induc ion of TR1 via Nrf 22mediated transcription.