Study on hydrogen peroxide induced DNA damage in myocardial H9c2 cells

Objective To study the effects of hydrogen peroxide on DNA damage and apoptosis in myocardial H9c2 cells line.Methods H9c2 cells were exposed to different concentration of H₂O₂(50,100,200,400μmol/L)in different time(1,3,6,12,24h).The cell viability was measured by MTT assay.Apoptosis was determined by AO/EB double fluorescent staining assay.The single cell gel electrophoresis(SCGE)was used to study the DNA damage of H9c2 cells.Results The experimental results demonstrated that H₂O₂ could damage H9c2 cells in dose dependant and time dependant way(P <0.05),and induce apoptosis with obviously morpho logical changes in exposed groups(P<0.05),especially at 6h,100 μmol/L level of H₂O₂,compared with NC(P<0.01).H₂O₂ could cause DNA damage.The rate of DNA damage was increased with the increase of dosage(P<0.01).Conclusion H₂O₂ could cause oxidative injury and DNA damage in H9c2 cells.Apoptosis and necrosis are main outcomes depending on exposure dose and exposure time.