Detection of fluorescent material in arsenic-resistant cells with LSCM

Objective To detect the fluore scentmaterial with in arsenic-resistant cells by lase rscanning confocalmicro scope(LSCM).Methods The arsenic-resistant cells were incubated with fluorescent dyes Rhodamine-123 for 30 minutes.The cells of test group were coincubated with Verapamil and NaSO₂,respectively.The cells of control group were merely administrated with Rhodamine 123.Fluo rescence miage acquisition of Rhodamine-123s' dynamic series were executed with LSCM,and the concentration fluorescence intracell at different time(12,24,36,48,64h)was recorded.Results After fluo rescence staining for 48h,the fluorescence intensity of the test group was 38.94±0.3648 and 38.153±0.533 for Verapamil and NaSO₂ treamtent.The intensities were significantly higher than that of the synchrono us control group.Conclusion The LSCM could be used to detect fluorescent material in arsenic resistant cells tmiely and quantitively.