Study on experimental conditions for phagocyteosis of mouse peritoneal macrophages

Objective To provide a reliable,sensitive and stable method for detecting the percentage of phagocytosis of mouse peritoneal macrophages.Methods The mouse peritoneal macrophages were incubated with latex beads in 24 well plate in cell culture incubator.The percentage of phagocytosis(PP) and phagocytosis index(PI) were detected by fluorescencem icroscope.Results As the culture tine increasing,PP of macrophages became higher (P<0.05).The PP of peritoneal macrophages increased with the increment of calf seaun concentration.As the concentration of latex beads increasing,the PP of peritonealm acrophages also increased(P<0.01).The PP of peritoneal macrophages of adherent macrophages was higher than that of non-adherentm acrophages(P<0.01).The PP ofperitonealmacrophages of the Balb/c micewas significandy higher than that of the C57,KM and ICR mice(P<0.01).Conclusion The optinal experinental conditions of the phagocyteosis of mouse peritoneal macrophages might be the concentration of latex beads of 1‰ the calf seaun concentration of 2.5%-5%,adding the latex beads and continuous culturing for 2 hours at the tin a of 2 hours after the adherence of macrophages Besides,it is indicated that Balb/c mouse is most suitable for detection of the capacity of phagocyteosis.