Surveillance of antibiotic susceptibility of Vibrio cholerae O1 and O139 in Guangdong province between 2006 and 2008

Objective To analyze antibiotic susceptibility of the V.cholerae strains isolated from clinical and environmental samples in Guangdong province during 2006 and 2008.Methods V.cholerae O1 and O 139(183 strains) from cholera cases (38 strains) and environmental samples(145 strains) in Guangdong province during 2006 and 2008 were colected The detection of ctxAB gene with PCR was employed to identify toxigenic and non-toxigenic strains Antibiotic susceptibility testing for the strains was perfouned on Muller-Hinton agar with disk diffusion method as recomm endid by CLSI (foamer NCCLS).Results There were 44 ctxAB gene positive strains in the 183 strains.Among the clinical and toxigenic strains, serotype O gawa showed resis-tance to tetracycline, nalidixic acid, and trinethoprin+sulfamethoxazo; while the serotype Indba showed a resistance rate of 100% to nalidixic acid and trinethoprin+sutfam ethoxazo; the O 139 show ed resistance to tetmcyc-line, nalidixic acid and trip ethoprin+sulfam ethoxazol The O gaw a and Inaba from environment and non-toxigenic serotype showed resistance to tetracycline, nalidixic acid and trinehtoprin+sulfam ethoxazol, and the O 139 strains resisted to nalidixic acid, trip ehtoprin+sutfamethoxazol and ampicilin.Most of clinical and toxigenic strains showed strongerm ultidrug resistance than that of the environm ental and non-toxigenic strains(P<0.01).The serotype Inaba from the clinical and toxigenic strains appeared NAL-SXT resistant, and most of the O 139 showed multidrug resistance to more than 3 antibiotics.On the contrary,most of serotypes of O gaw a and Inaba from environmental and non-toxigenic strains showed multidmg resistance to more than 3 antibiotics, while the most of the O 139 displayed resistant to NAL AM P and SXT AM P.Conclusion Most of clinical and toxigenic V.cholerae strains isolated in Guangdong province showed strong multidrug resistance than environmental and non-toxigenic strains.It is important to survey the multidrug resistance of the V.cholerae to control cholera efficiently.