Effect of praziquantel, albendazole and mebendazole on recombinant lactate dehydrogenase of Taenia asiatica

Objective To detect the effect of praziquantel,albendazole,and mebendazole on activity of the recom binat lactate dehyd rogenase of Taenia asiatica(rTaLDH).Methods The effects of praziquantel,a lbendazole,and mebendazole on the enzymatic activity of rTaLDH were detected by spectrophotometric method in standard reaction systemes tablished before.The solvent of each reagent was used as control.The results were analyzed with SPSS16 10 softw are.Results Compared with the control,considerab le inhibition for both reduction and oxidation reactions catalyzed by rTaLDH was observed at 0.10mmol/L of praziquantel(P<0.01).Compared with the control,the enzyme activities of rTaLDH for reduction and oxidation reactions were inhibited by 93.99% and 94.67%,respectively,at 0.1 mmol/L of praziquantel(P<0.01).At 0.15mmol/L of mebendazole,enzymatic activities were inhibited by 92.2% for reduction reaction and 86.13% for oxidation reac tion,respectively,lower than that of the control(P<0.01).Conclusion Praziquantel shows a high inhibition on rTaLDH.TaLDH may be one of the molecular targets of praziquantel,albendazole,and mebendazole.