Multi-gene risk analysis of susceptibility genes on primary gastric cancer

Objective To screen and assess genetic risk factors(gene polymorphism) of primary gastric cancer susceptibility and to select the genotype relating to gastric cancer in Han population of Nanjing city.Methods A 1:1 matched case-control study was carried out in 374 primary stomach cancer cases(273 males and 101 females) and gender,age matched controls in Nanjing city.PCR-RFLP and AS-PCR technologies were used to determine the genotypes of CYP2E1,GSTM1,GSTT1,NAT2,ALDH2,MTHFR,XRCC1,IL-1β,VDR,and TNF.Conditional-logistic regression model was adopted to screen the environmental and genetic risk factors of primary gastric cancer and to select the genotype relating to gastric cancer for multi-gene risk analysis.Results The genetic risk factors associated to primary gastric cancer susceptibility were CYP2E1 wild genotype(odds ratioOR=1.348,95% confidence intervalCI=1.075-1.690),NAT2M1 mutant genotype(OR=2.310,95%CI=1.613-3.309),NAT2 slow-acetylating genotype(OR=1.768,95%CI=1.018-3.072),XRCC1 194 mutant genotype(OR=1.449,95%CI=1.140-1.842),MTHFR A1298C mutant genotype(OR=1.521,95%CI=1.11-2.083),IL-1β mutant genotype(OR=1.271,95%CI=1.031-1.568).Multi-gene risk analysis showed that there was a close relation between the OR of polygenic combination and genotypes frequency.Conclusion With the increase of the susceptibility gene,the risk of primary gastric cancer increases.Multi-gene risk assessment can further promote the recognition of susceptible population.