Effect of protoporphyrin disodium on oxidative enzyme in liver tissue of mice with acute hepatic injury

Objective To study the effect of protoporphyrin disodium(NAPP)on activities of catalase(CAT)and glutathione peroxidase(GSH-Px)in livertissue of mice with acutehepatic injury induced by carbon tetrachloride(CCl₄).Methods Sixty ICR mice were randomly divided into normal control,model,bifendate pills,and low,medium,high dose NAPP group.The mice in each treamtent group were treat with different drugs by intraga stricadmin istration continuously and then a single dose of CCl₄ was given to establish acute hepatic injury model.Six teen hours later,the liver was collceted to observe histo pathology and calculate liver index.Then the liver homogenate was prepared to detect the activities of CAT and GSH-Px.Results Compared with the normal control group,the activities of CAT and GSH-Px were 19.67±4.46 and 193.39±42.36 U/mg·prot in liver tissue with a significant difference(P<0.01).The activities of CAT and GSH-Px in the liver tissues of the bifendate pills group,low,medium,and high dose NAPP group were 29.98±4.79,27.74±6.11,28.13±5.67,33.82±6.00 U/mg·prot,and 265.72±48.42,200.24±39.30,231.73±46.70,257.64±37.11 U/mg·prot,respectively,with statistical differences compared with those of model group(P<0.05).Hepatic histopathology detection showed that NAPP effective ly alleviated the hepatic injury induced by CCl₄,and the slightest in jury was observed in high dose NAPP group.Conclusion NAPP has anti-oxidative effect and can effectively prevent the decrease of CAT and GSH-Px activities in liver tissue of mice with acute hepatic in jury induced by CCl₄.