Serum reproductive hormone and expression of apoptosis gene in spermatogenic cell in rats with manganese exposure

Objective To study there lation ship bewteen serumreproductive horm one level and expression of caspase-3 in sperma to genic cell in rats with manganese exposure.Methods Forty eight male rats were randomly divided into one control group and two mang anese chloride(MnCl₂)(15,30 mg/kg)exposure groups.After exposed to manganese for 4 and 6 weeks,the serum testoste rone(T),follicle stmiulating bormone(FSH),and luteinizine hormone(LH)levels were detected with radiomimunoassay.The expression of caspase 3 in spermatogenic cells was detected with mimunoh isto chemical methods (SABC).Results The levels of T,FSH and LH in 30 mg/kg MnCl₂ groups were 2.78±0.74 nmol/L,0.44±0.03 U/L,0.19±0.02 U/L for rats wtih 4 weeks exposure and 2.76±0.81 nmol/L,0.57±0.03 U/L,0.19±0.02 U/L for rats with 6 weeks exposure,respectively.The percentages of caspase-3 positive cell were 65.49±11.34 and 82.65±5.26 for rats with 4 weeks and 6 weeks of mangan sesexposure,respectively.There existed anegative correlation between the caspase-3 positive cell rate and serum level of T(r=0.799,P<0.01).There existed a positive correlation bewteen the caspase-3 positive cell rate and serum levels of FSH and LH(r=0.735,P<0.01;r=0.737,P<0.01).Conclusion The decrease of serum T and the increases of serum FSH and LH may be the miportant causes of the up regulation of caspase-3 expression in spermatogenic cells in rats exposed to manganese.