Analysis of genotype and antibody level of mumps virus among healthy people in Shaanxi province

Objective To study the gene distribution and the an tibody level of mumps virus among healthy people in Shaanxi province.Methods Specimens of throat swabs from mumps patients were collected for isolation of mumps virus. The gene fragment of 316 nucleotides coding small hydro-phobic protein was amplified with RT-PCR method and its gene sequence and genotype was analyzed.The IgG antibody level of mumps virus among healthy people was deter mined with ELISA method.Results Sixstrains of mumps virus were isolated from specmiens collected.All viruses isolated belonged to Fgene group of mumps virus.The positive rate of mumps virus antibody was 66.08%.The content and positive rate of IgG antibody were the lowest among small children younger than 1.5 years(236.68 U/ml,37.04%)and increased with the in crement of age.There was no significant difference in content and positive rate of antibody between the boys and girls.Con clusion Mumpsep idemics in Shaanxi province were caused by F genotype mumps virus.The antibody level aga in stmumps virus among healthy children in Shaanxi province is low.