Effects of bFGF on expression of c-Myc in cerebral tissue during focal cerebral ischemia in rats

ObjectiveTo investigate the expression of c-Myc in hippocampus and cortex after cerebral ischemia and reperfusionin rats and to explore regulative effects and mechanism of basic fibrob last growth factor(bFGF)to c-Myc in brain tissue.MethodsThe model of middle cerebralartery occlusion(MCAO)was established with intraluminal filament blockade.The expression of c-Myc and cell apoptosis in hippocampus and cortex were detected with mimunohisto chemical and terminal deoxyneucleo tidyl transferase dUTP nick end labeling(TUNEL)method.ResultsNo apoptotic cell was observed in sham-operation group.The expression of c-Myc in the cortex and hippocampus tissue of rats was at low level in the sham-operation group.In ischemia-reperfusion group,the apoptotic neurons in ischemic-region of cortex and hippocampus were enhanced.The decrease of apoptotic neurons inischemic region of cortex and hippocampus was observed in bFGF treamtent group.The gray scale value of the expression of c-Myc was increased in the model group(88.16±2.43, 86.72±1.23).The gray scale value of the expression of c-Myc in the cortex and hippocampus tissue was markably decreased in the treatment group than that of in the model group(97.61±1.78,95.35±2.34).ConclusionThe results indicate that bFGF depress cell apoptosi,sparticipate in the regulation of expression of c-Myc inischemic neurons.