Characterization and molecular typing of suspected Vibrio cholerae isolates

Objective To identify two suspected Vibrio cholerae isolates from patients with intestinal diseases diagnosed in a hospital in Beijing city and to detect their main virulence genes and analyze their genotypes.Methods The suspected V.cholerae isolates were identified with API 20E strips,serotype and 16S rRNA analysis.PCR was used to detect the distribution of the eight major virulence genes,and the genetic association was measured with pulsed-field gel electrophoresis(PFGE).Results The two suspected V.cholerae isolates were confirmed as non-O1/non-O139 V.cholerae.16SrRNA analysis showed that the two isolates was 100% homologous to those from NCB I database.Virulence geneshy Al, omp W and toxR were detected.The two strains showed different banding patterns with PFGE.Conclusion The two suspected strains were identified to be non-O 1/non-O 139 V.cholera and the pathogenic factors may be related to the virulence geneshy Al,ompW and to xR.The two strains were distant in evolutionary relationship.The result indicates that different types of non-O 1/non-O 139 V.cholera exist in the city.