Effects of iodine on phenotype and function of murine dendritic cells in mice

Objective To discuss the influence of dietaryiodine intake on the phenotype and function of murine dendritic cells(DL s).Methods Forty female C57BL/6J mice were randomly divided in to four groups according to the iodine in take:lowiodine(LI), normal iodine(NI), 10 folds excessiveiodine(10 HI), and 50 folds excessive iodine(50HI).Eightmonths after the treamtent, the mice were sacrificed and the mononuclear cells were isolated from bone marrowof the mice and cultured in RPM Imedium 1640 containing differentcy to kines to generate DCs.The phenotypic molecules, the level of inter leuk in 12(IL-12) in the supernatan to fcultures of DCs and the proliferation of allogenic T-cells stimulated by DCs were analyzed.Results The percentages of CD40⁺, CD 80⁺, CD 86⁺ and major histocom patibility complex class Ⅱ⁺ cells in CD11c⁺ cells of LI were significantly lower than those of NI.Except the percentage of CD 40⁺ cells in CD11c⁺ cells, all the parameters were statistically increased in 50H I compared with the NI(P < 0.05 or P < 0.01).The level of IL-12 in the supernatant of culture of DCs in LI was considerably decreased(P < 0.01), while that of 50H I was significantly enhanced compared with the NI(P < 0.01).Stmiulative index(SI) of LI was 5.23±1.96, which was statistically lower than that of the NI(8.61±2.15, P < 0.05).SI of 50H I(12.38±2.61) wasmuch higher than that of the NI.There was no difference in SI between 10HI and NI.Conclusion The results demonstrate that high level of iodine intake could promote differentiation and maturation of murine dendritic cells and enhance the mimune function in vivo.