Function and prokaryotic expression of arginyl-tRNA-protein transferase in Teania, saginata

Objective To predict structural characte ristics of arginyl tRNA prote in transferase in Teania.saginata by bioinformatics and to detectits prokaryotic expression.Methods By online analysis with bioinform atics websites and soft ware package, the function of arginyl tRNA protein tranferase gene was predicted and the gene was inserted into the prokary otic expression vectors pET 28a(+) and amplified with PCR and its sequence was determined.Results Anovel cDNAse quence encoding arginyl tRN A protein transferase with a molecular weight of 56436 3 was identified.The cDNA is 1476bp and codes 491 amino acids.PCR, double enzyme digestion and DNA sequencing indicated that pET 28a(+) and arginyl RtNA prote in transferase recombinant plasmid were successfully constructed.Sodium dodecyl sulfate poly acry lamide gelelectrophoresis(SDS PAGE) results showed that the gene expressed in Escherichia coli BL 21/DE3.Conclusion Anovel gene of Teania.saginata is successfully identified and expressed by prokaryotic vectors.