Detection on promoter methylation of c-myc gene in thymic lymphomas induced by ionizing radiation

Objective To study methy lation of CpGislands in prom oter region of c-mcy gene and the change of mRNA expression in thymic lymphomas of mice induced byionizing radiation.Methods The thymic lymphom as model was made with X-ray exposure in BAL B/c mice.The methylation of CpG islands in prom oter region and the mRNA expression of c-myc gene were detected with bisulfite sequencing PCR and RT-PCR.Results CpG islands inprom oter region of c-myc gene were at demethy lation state in thymic lymhom as induced by ionizing radiation against normal thymus.The mRNA expression of c-myc gene in thymic lymphomas(1.12±0.99) was higher than that of in normal thym us tissue(0.89±0.08) with a tatistical sign if icance(P < 0.01).Conclusion There is a corre lation between promoter demethylation of c-myc gene and thymiclym phom as induced by ionizing radiation.