Bioinformatics analysis of function and prokaryotic expression of elongation factor-1 in Taenia. solium

Objective To analyze and predict structural charac teristics of elongation factor1 in Teania.solium with bio informatics and to detectits prokaryotic expression.Methods Byonline analysis with bioinformatics websites and soft ware package, the physical chemical charac teristics, modification sites after translation, domains, subcelluar location,topological structure, second structure, and 3D structure of elong ation factor 1(EF1) were ana lyzed.The EF1 gene was inserted in to the prokaryotic expression vector pET 28a(+) and then was am plified with PCR and sequenced.Results Anovel cDNA sequence EF1 with 1657 pbcoding 488 aminoacids and a molecular weight of 49011.5 was identified.PCR,double enzyme digestion and DNA sequencing indicated that pET 28a(+) and EF 1 recom binant plasmid were succe ssfully constructed.Sodium dodecyl sulfate poly aery lamide gelelectrophoresis(SDS PAG E) results showed that the gene was expressed in E scherichia coli BL21/DE3.Conclusion A novelgene of Taenia.solium is successfully identified and expressed with prokaryotic vector.