Genetic characterization of wild-type rubella virus isolated in Liaoning province

Objective To analyze genetic characterization of rubella virusiso lated in Liaoning province and to provide scientific basis for rubella control and elmiination.Methods Vero/Slam cells were used for rubella virus isolation and 22 rubella viruses were isolated from the specmiens collected from rubella outbreaks and sporadic patients during 2007-2008.The fragments containing 739 nucleotides of E1 genes from 22 rubella virus isolate swere amplified with RT PCR method.The PCR products were sequenced and analyzed with WHO rubella reference strains down loaded from Genbank.Results The results of homology and phylogenetic analyses based on 739 nucleotides showed that 22 rubella virus strains belong to 1E geno type.Twenty two strains showed a nucleotide acids and amino acid smiilarity of 97.6% 100.0% and 99,1% 100,0%.Compared to the 1E reference strains,nucleotide acids and amino acid similarity were 97.0% 99.1% and 99.1% 100.0%.Conclusion The results suggest that 1E genotype of rubella virus is the predom inance geno type in Liaoning province.