Effects of high temperature combined with endotoxin on apoptosis of RAW264.7 cell

Objective To study the effect of high temperature combined with endotoxin on the apoptosis of RAW 264.7 cell.Methods The cultured RAW 264.7 cells in vitro were divided into 4 groups:normal temperature(37℃) blank,high temperature(40℃) blank,37℃+lipopoly saccharide(LPS),and 40℃+LPS guoup.After 3 and 24 hours culture under the condition of 95% O₂ and 5% CO₂,we detected the apoptoisis rate of RAW 264.7 cells with flow cytometry.Results Apoptosis rates were extremely low in 37℃ blank group(both 3hr and 24hr),as well as in 37℃+LPS group.The high temperature(40℃) groups,on the other hand,showed higher possibilities of cell apoptosis.Compared with 37℃+LPS goups,40℃+LPS group showed a much more obvious tendency of cell apoptosis(P < 0.05).But there was no significant difference(P > 0.05) between the apoptosis rate of 40℃ blank group and that of 37℃ group.As time passed by,the rate of cell apoptosis increased markedly (P < 0.05).Conclusion High temperature combined with endotoxin plays an importantrole in the apoptosis of RAW 264.7 cell.And the severity of cell apoptosis rises significantly with the extension of exposure tmie.