Effects of carbon disulfide on adhesion molecule of mice in different reproductive phases

Objective To study the influence of carbon disulfide(CS₂) on the expression of adhesion molecule in embryo and uterus tissues of mice in different reproductive phases.Methods The female mice were randomly divided into the groups of follicular development and embryo implantation,which were subdivided into early-and later-implantation phases,and intraperitoneally injected with CS₂ at dose of 631.4 mg/kg for two successive days.All samples were collected on the 9th day of gestation.The expression of adhesion molecule fibronectin(FN) and in tegrin β₁ in embryo and uterus tissues were detected with sodium dodecy l sulfate-polyacrylam ide gelelectrophoresis(SDS-PAGE) and western blotting.Results With CS₂ exposure at the phase of embryo implantation,the expression of FN and integrin β₁ in embryo and uterus tissues were decreased by 54.8%,10.4%,52.5%,and 21.1%,and the differences were statistically significant(P < 0.05 for all).With CS₂ exposure at the phase of follicular development,the expression of FN and integrin β₁ in uterus tissue were decreased by 69.4% and 16.0% (P < 0.05),and the expression of FN in embryo tissue was decreased by 44.6% (P < 0.01).But the reduction of integrin β₁ in embryo tissue was not statistically significant(P > 0.05).The number of conception was significantly reduced by 37.5% with CS₂ exposure at the phase of embryo implantation(P < 0.01).Whereas with CS₂ exposure in the follicular development phase,the number was decreased slightly (P > 0.05).Conclusion CS₂ could reduce the expression of adhesion molecule FN and integrin β₁ in embryo and uterus tissue of pregnant mice,and the reduction of adhesion molecule is related to implantation failure.