Changes of angiotensin in diabetic rats with myocardial ischemic/reperfusion injury

Objective To analyze the effect of Herba Cichorii glycocalyx on angiotensin in streptozotocin-induced diabetic rats with myocardial ischemic/reperfusion injury.Methods The diabetic model of rats was established by intraperitioneal injection of streptozotocin.Twelve weeks later,trachea cannula and installation of life-support machine were performed under the condition of anaesthesia with intraperitioneal injection of pentobarbitol sodium sixty minutes after the ligation,the left anterior desending coronary artery was loosened for a sixty minutes reperfusion.The changes of electrocardiogram in Ⅱlead during whole course of experiment were recorded.Sixty minutes after the reperfusion,blood samples of the rats were collected for detections of creatine kinase(CK),lactic dehydrogenase(LDH),angiotensin I(Ang I),and angiotensin Ⅱ(Ang Ⅱ).Results The level of CK,LDH,AngⅠ,and AngⅡ in the diabetic rats were 5.98 U/ml,10.03 U/ml,790.4 ng/L,and 1 773.7 ng/L,while the level of CK,LDH,AngⅠ,and AngⅡ in the rats with chicory polysaccharide intervention were 1.39 U/ml,7.35 U/ml,443.9 ng/L,and 1 552.2 ng/L,respectively,and significantly lower than those in diabetes model(P<0.01 for all).Conclusion The chicory polysaccharide has protective effect on myocardial injury induced by ischemic/reperfusion in streptozotocin-induced diabetic rats through decreasing production of Ang Ⅰ and Ang Ⅱ.