Subchronic cadmium exposure induces renal lipid peroxidation injuries in WZSP

Objective To study the effect of subchronic cadmium(Cd) exposure on renal lipid peroxidation reaction in Wuzhishan pig and to explore the mechanism of subchronic cadmium exposure on renal injury.Methods Sixteen healthy Wuzhishan pig of 3-month-old were fed with basal diet that contains 0(control group),0.5(low dose group),8.0(midium dose group),and 32.0 mg/kg(high dose group) cadmium for 100 days.The kidneys of the pigs were collected at the end of the treatment to determine glutathione peroxidase(GSH-Px),total superoxide dismutase(SOD),catalase(CAT) activities,and malondialdehyde(MDA) contents,and the body weight and organ coefficient of the pigs were also determined.Results Compared with the control group,the body weight of low dose group(37.3±1.8 kg) increased significantly(P<0.05).CAT activities in low,moderate,and high Cd exposure group were 14.1±3.2,17.1±1.3,and 14.5±1.1 U/mg·prot,respectively,with significant increases compared with the control group(10.8±1.8 U/mg·prot,P<0.05).Total SOD,GSH-Px activity and MDA content showed no significant differences between different groups(P>0.05).ConclusionSubchronic cadmium exposure less than the concentration of 32.0 mg/kg does not induce renal lipid peroxidation injuries in WZSP.The antioxidant system of renal cortex may involved in mechanisms of cadmium nephrotoxicity.