Anticoagulant effect of total diterpenoids in Isodon japonica(Burm.f.) Hara var.glaucocalyx(Maxim.) Hara in mice

Objective To explore anticoagulant effect of total diterpenoids extracted with ethanol from Isodon japonica(Burm.f.) Hara var.glaucocalyx(Maxim.) Hara in mice.Methods A total of 90 mice were randomly divided into blank control group,positive drug group,and high,medium,low dose total diterpenoids groups.The different treatments were conducted with gavage administration once a day for 7 days.Tail transection and capillary glass tube method were used to determine clotting time(CT) and bleeding time(BT).Results The CT was 425.00±13.32 seconds and 559±36.17 seconds for the mice treated with high dose total diterpenoids extracted with heating reflux and cold soak method,respectively,and significantly longer than that of the blank control(201.00±18.52 seconds).The BT was 3308.00±271.33 seconds and 3758.00±184.53 seconds for the mice treated with high dose total diterpenoids extracted with heating reflux and cold soak method,respectively,and significantly longer than that of blank control(2094.00±58.59 seconds).Conclusion Total diterpenoids extrated from Isodon japonica(Burm.f.) Hara var.glaucocalyx(Maxim.) Hara could extend capillary blood coagulation time and tail bleeding time and has anticoagulant effect in mice.