Effect of C/EBP gene on proliferation,apoptosis and migration of HeLa cells

ObjectiveTo explore the effect of CCAAT/enhancer binding protein β(C/EBPβ) gene on proliferation,apoptosis,and migration of HeLa cells.MethodsThe HeLa cells were cultured and assigned into C/EBPβ vector pcDNA3.1 transfection group,empty vector pcDNA3.1 transfection group,and non-transfection group.The proliferation of the HeLa cells was determined with 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide(MTT) assay.The apoptosis of the HeLa cells was detected with annexin Ⅴ/PI double staining flow cytometry assay.The migration of HeLa cells was measured with Transwell assay,in which the upper chambers containing C/EBPβ vector transfection,empty transfection,and non-transfection HeLa cells and the lower chambers containing 20% phosphate buffer solution culture medium.ResultsThe absorbance value of C/EBPβ gene vector transfection,empty vector transfection,and non-transfection HeLa cells group 0.38±0.12,0.59±0.17,and 0.68±0.17,respectively,with significant differences among the groups(P<0.01).The apoptosis rate of C/EBPβ gene vector transfection,empty vector transfection,and non-transfection HeLa cells were 6.99±0.75,3.48±0.74,and 3.23±1.07,respectively,with significant differences among the three groups(P<0.01).The number of C/EBPβ gene vector transfection HeLa cells penetrating through the membrane were significantly reduced compared with those empty vector transfection and non-transfection HeLa cells(16±2.3 vs 39±3.2 and 39.6±2.6).ConclusionC/EBP β gene can inhibit proliferation and migration of HeLa cells and promote the apoptosis of the cells.