Association of eNOS gene rs7830 and rs3918188 with EH and its risk factors among Han in Xinjiang

Objective To explore the association of endothelial nitric oxide synthase(eNOS) gene rs7830 and rs3918188 polymorphism with essential hypertension(EH) and its risk factors among Han in Xinjiang Uygur Autonomous Region.Methods Based on the results of previous epidemiological investigation,346 EH patients(EH group) and 385 non-EH patients(NT group) were selected among Han residents in Xinjiang in 2007-2009 .DNA was extracted with phenol/chloroform extraction.SNaPshot technique was adopted for the detection of polymorphism.The correlation of polymorphism with EH and its risk factors were analyzed.Results Age,body mass index were the risk factors for EH(P<0.05) among the subjects.The distribution frequency of eNOS rs7830 loci CC,AC,and AA genotype was 36.4%,53.5%,and 10.1% for EH group and 37.7%,44.9%,and 17.4% for NT group with statistically significant differences(P<0.05 for all) .The frequency of eNOS rs 7830 C and A allele was 63.2% and 36.8% for EH group,and 60.1% and 39.9% for NT group,without significant difference(P>0.05).The distribution frequency of eNOS rs3918188 loci CC,CA,and AA genotype was 47.7%,44.8%,and 7.5% for EH group and 41.3%,46.5%,and 12.2% for NT group,without significant differences(P>0.05 for all) .The frequency of eNOS rs 3918188 C and A allele was 70.1% and 29.9% for EH group,and 64.5% and 35.5% for NT group,without significant differences(P>0.05).The frequency of eNOS gene rs7830 and rs3918188 CC haplotype was 34.11% and 27.52%for EH group and NT group,with a significant difference(P<0.05) between the two groups.The risk of EH prevalence for CC haplotypes was 1.363.Conclusion Age,body mass index are EH risk factors among Han in Xinjiang.The eNOS gene rs3918188 and rs7830 loci polymorphism maybe associate with EH among Han in Xinjiang.