Effects of anthocyanin on BCL2 family of platelet apoptosis pathway

Objective To investigate the effects of anthocyanin cyanidin-3-glucoside (Cy-3-g)on BCL2 family of platelet apoptosis pathway.Methods Peripheral venous blood samples of healthy volunteers were collected and purified platelet suspension was prepared.Gel-filtered platelets were pre-incubated with 0.5,5,and 50 μmol/L Cy-3-g and induced by collagen.Flow cytometry was used to measure platelet apoptosis rate and total platelet proteins were extracted;the expressions of anti-apoptotic proteins BCL-XL and pro-apoptotic proteins BAK,BAX,BID and cleaved BID were quantified with western blot.Results Compared with the control group,5 μmol/L Cy-3-g significantly increased the rate of platelet apoptosis and 5 μmol/L (0.739±0.047),50 μmol/L(0.884±0.004)Cy-3-g effectively reduced expression of anti-apoptotic protein BCL-XL in platelets(P<0.01).Compared with the control group,5,50 μmol/L Cy-3-g significantly promoted the expressions of pro-apoptotic proteins BAK,BAX,BID and the differences were of statistic significance at concentrations of 5 μmol/L(1.667±0.063,2.271±0.165,1.861±0.231)and 50 μmol/L (1.531±0.039,1.895±0.018,1.311±0.121),respectively(all P<0.05).Conclusion Cy-3-g effectively promotes platelet apoptosis by affecting the proteins expression of BCL2 family.